RESUMO
Cyp19a1a is a key gene responsible for the production of estradiol-17ß (E2), the main functional estrogen and a major downstream regulator of reproduction in teleost fish. It is widely known that CYP19 gene expression, aromatase activity, and E2 production can influence gonadal differentiation and sex reversal in teleost fish, but the feedback mechanisms whereby E2 regulates cyp19a1a remain poorly understood, especially regarding the potential roles of endogenous small RNA molecules (miRNAs). Here, we identified miR-26a-5p as a regulatory factor of its predicted target gene (cyp19a1a). In vitro and in vivo studies showed that miR-26a-5p can decrease cyp19a1a expression. Furthermore, high doses of E2 act as a repressor of miR-26a-5p. This study proposes a regulatory feedback loop whereby E2 regulates cyp19a1a through miR-26a-5p, and suggests that this positive feedback is an important aspect of the control of E2 production.
Assuntos
Aromatase/genética , Bass , Transtornos do Desenvolvimento Sexual , Estradiol/farmacologia , MicroRNAs/genética , Animais , Aromatase/metabolismo , Bass/genética , Bass/metabolismo , Transtornos do Desenvolvimento Sexual/genética , Transtornos do Desenvolvimento Sexual/metabolismo , Retroalimentação Fisiológica/efeitos dos fármacos , Feminino , Gônadas/efeitos dos fármacos , Gônadas/metabolismo , Gônadas/fisiologia , Masculino , MicroRNAs/metabolismo , Processos de Determinação Sexual/efeitos dos fármacos , Processos de Determinação Sexual/genética , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/genéticaRESUMO
In our previous studies, diploid and triploid hybrids have been detected from the hybridization of Epinephelus coioidesââ¯×â¯E. lanceolatusâ. The triploid groupers have been found to be delayed in gonadal development, but the mechanism remains poorly understood. In this study, we examined the gonadal development, assayed the serum steroid hormone levels, and compared the BP (brain and pituitary) and G (gonad) transcriptomes of 18-month-old diploid and triploid hybrids. The results showed that levels of serum estradiol-17ß and testosterone were significantly higher in triploid groupers. The RNA-seq data revealed that 1518 and 14,963 differentially expressed genes were identified in the BP and G transcriptome, respectively. Further analysis revealed that the expression levels of genes involved in the sexual differentiation pathway and sex steroid synthesis pathway are significantly higher in triploid hybrids. Our findings provided a comprehensive insight into a better understanding of the regulatory mechanisms of sterility in triploid hybrid fish.
Assuntos
Bass/genética , Gônadas/crescimento & desenvolvimento , Diferenciação Sexual , Transcriptoma , Triploidia , Animais , Bass/crescimento & desenvolvimento , Estradiol/metabolismo , Feminino , Gônadas/metabolismo , Hibridização Genética , Masculino , Testosterona/metabolismoRESUMO
The complete mitochondrial genome of the hybrid grouper Epinephelus coioides â × Epinephelus akaara â is presented in this study. The mitochondrial genome is 16,458bp long and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. The gene order and composition of the hybrid grouper mitochondrial genome was similar to that of most other vertebrates. The nucleotide compositions of the light strand in descending order is 28.87% of G, 28.26% of T, 26.58% of A and 15.79% of C. With the exception of the NADH dehydrogenase subunit 6 (ND6) and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand. The phylogenetic analysis by maximum-likelihood (ML) method showed that the hybrid grouper has the closer relationship to the hybrid grouper Epinephelus coioides â × Epinephelus lanceolatus â.
RESUMO
Grouper (Epinephelus spp.) is a group of fish species with great economic importance in Asian countries. A novel hybrid grouper, generated by us and called the Hulong grouper (Hyb), has better growth performance than its parents, E. fuscoguttatus (Efu, â) and E. lanceolatus (Ela, â). We previously reported that the GH/IGF (growth hormone/insulin-like growth factor) system in the brain and liver contributed to the superior growth of the Hyb. In this study, using transcriptome sequencing (RNA-seq) and quantitative real-time PCR (qRT-PCR), we analyzed RNA expression levels of comprehensive genes in the muscle of the hybrid and its parents. Our data showed that genes involved in glycolysis and calcium signaling in addition to troponins are up-regulated in the Hyb. The results suggested that the activity of the upstream GH/IGF system in the brain and liver, along with the up-regulated glycolytic genes as well as ryanodine receptors (RyRs) and troponins related to the calcium signaling pathway in muscle, led to enhanced growth in the hybrid grouper. Muscle contraction inducing growth could be the major contributor to the growth superiority in our novel hybrid grouper, which may be a common mechanism for hybrid superiority in fishes.
Assuntos
Bass/crescimento & desenvolvimento , Bass/genética , Proteínas de Peixes/genética , Vigor Híbrido/genética , Músculos/metabolismo , Transcriptoma/genética , Animais , Reação em Cadeia da Polimerase em Tempo RealRESUMO
The Asian arowana (Scleropages formosus), one of the world's most expensive cultivated ornamental fishes, is an endangered species. It represents an ancient lineage of teleosts: the Osteoglossomorpha. Here, we provide a high-quality chromosome-level reference genome of a female golden-variety arowana using a combination of deep shotgun sequencing and high-resolution linkage mapping. In addition, we have also generated two draft genome assemblies for the red and green varieties. Phylogenomic analysis supports a sister group relationship between Osteoglossomorpha (bonytongues) and Elopomorpha (eels and relatives), with the two clades together forming a sister group of Clupeocephala which includes all the remaining teleosts. The arowana genome retains the full complement of eight Hox clusters unlike the African butterfly fish (Pantodon buchholzi), another bonytongue fish, which possess only five Hox clusters. Differential gene expression among three varieties provides insights into the genetic basis of colour variation. A potential heterogametic sex chromosome is identified in the female arowana karyotype, suggesting that the sex is determined by a ZW/ZZ sex chromosomal system. The high-quality reference genome of the golden arowana and the draft assemblies of the red and green varieties are valuable resources for understanding the biology, adaptation and behaviour of Asian arowanas.
Assuntos
Evolução Molecular , Peixes/genética , Genoma , Filogenia , Animais , Feminino , Repetições de Microssatélites/genética , Cromossomos Sexuais/genéticaRESUMO
The complete mitochondrial genome of the hybrid grouper (Epinephelus coioidesâ × Epinephelus lanceolatusâ) was presented in this study. The mitochondrial genome is 16,743 bp long and consists of 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and a control region. The gene order and the composition of the hybrid grouper mitochondrial genome were similar to that of most other vertebrates. The nucleotide compositions of the light strand are 26.56% of A, 15.79% of C, 28.74% of T, and 28.91% of G. With the exception of the NADH dehydrogenase subunit 6 (ND6) and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand.
Assuntos
Bass/genética , Quimera/genética , Mitocôndrias/genética , Análise de Sequência de DNA/métodos , Animais , Composição de Bases , Feminino , Ordem dos Genes , Tamanho do Genoma , Genoma Mitocondrial , Masculino , Fases de Leitura Aberta , RNA Ribossômico/genética , RNA de Transferência/genéticaRESUMO
The complete mitochondrial genome of the Epinephelus akaara was presented in this study. The mitochondrial genome is 16,743 bp long and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. The gene order and composition of Epinephelus akaara mitochondrial genome was similar to that of most other vertebrates. The nucleotide compositions of the light strand are 27.31% of A, 16.20% of C, 28.68% of T and 27.81% of G. With the exception of the NADH dehydrogenase subunit 6 (ND6) and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand.
Assuntos
Bass/genética , Genoma Mitocondrial , Animais , Composição de Bases/genética , Sequência de Bases , Mapeamento Cromossômico , DNA Mitocondrial/genética , Genes Mitocondriais , RNA Ribossômico/genética , RNA de Transferência/genéticaRESUMO
The complete mitochondrial genome of the hybrid grouper Epinehelus moara â × Epinephelus lanceolatus â is presented in this study. The mitochondrial genome is 16,418 bp long and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. The gene order and composition of the hybrid grouper mitochondrial genome were similar to that of most other vertebrates. The nucleotide compositions of the light strand in descending order was 28.86% of G, 28.61% of T, 26.46% of A and 16.08% of C. With the exception of the NADH dehydrogenase subunit 6 (ND6) and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand. The phylogenetic analysis by maximum-likelihood (ML) method shows that the hybrid grouper has closer relationship to Epinehelus moara and Epinehelus bruneus.
RESUMO
The complete mitochondrial genome of the Epinephelus awoara was presented in this study. The mitochondrial genome is 16 798-bp long and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region. The gene order and composition of Epinephelus awoara mitochondrial genome was similar to that of most other vertebrates. The nucleotide compositions of the light strand in descending order is 27.35% of A, 16.53% of C, 28.44% of T, and 27.69% of G. With the exception of the NADH dehydrogenase subunit 6 (ND6) and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand. The phylogenetic analysis by maximum-likelihood (ML) method shows that the Epinephelus awoara was closer to Epinephelus fasciatomaculosus in the phylogenetic relationship.
Assuntos
DNA Mitocondrial/genética , Genoma Mitocondrial/genética , Perciformes/genética , Animais , Composição de Bases/genética , Sequência de Bases/genética , Sequência Conservada/genética , Ordem dos Genes/genética , Genes Mitocondriais/genética , Filogenia , Análise de Sequência de DNA/métodosRESUMO
The complete mitochondrial genome of the Epinephelus fuscoguttatus was presented in this study. The mitochondrial genome is 16,648 bp long and consists of 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and a control region. The gene order and the composition of E. fuscoguttatus mitochondrial genome were similar to that of most other vertebrates. The nucleotide compositions of the light strand are 26.91% of A, 15.58% of C, 29.19% of T, and 28.32% of G. With the exception of the NADH dehydrogenase subunit 6 (ND6) and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand.
Assuntos
Bass/genética , Genes Mitocondriais/genética , Genoma Mitocondrial/genética , Perciformes/genética , Animais , DNA Mitocondrial/genética , Ordem dos Genes/genética , Genes de RNAr/genética , Filogenia , RNA de Transferência/genética , Análise de Sequência de DNA/métodosRESUMO
We presented the complete mitochondrial genome of the Epinephelus moara in this study. The mitochondrial genome is 16,696 bp long and consists of 13 protein-coding genes, two rRNA genes, 22 tRNA genes and a control region. The gene order and composition of Epinephelus tukula mitochondrial genome was similar to that of most other vertebrates. The nucleotide compositions of the light strand are 26.45% of A, 16.09% of C, 28.60% of T and 28.86% of G. With the exception of the NADH dehydrogenase subunit 6 (ND6) and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand.
